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Diagnostic reagents
Additives for diagnostic reagents
NOF is developing Biolipidure® series and LIPIDURE®-SF series as additives for in vitro diagnostics.
Function of LIPIDURE®
As additives for diagnostics, Biolipidure® has multiple functions such as suppression of non-specific adsorption of protein, stabilization of protein, and sensitization, all of which are required for developing diagnostic reagents.
Basic function of Biolipidure®
The table below shows basic functions of Biolipidure® as additives for diagnostic reagents.
The Biolipidure® series has great variety of polymers having different physical properties, which is derived from rich variation of secondary monomers copolymerized with MPC.
Biolipidure®- | Side chain property | Blocking effect | Stabilizing effect | Sensitizing effect | Improvement of reproducibility and accuracy | Solubilizing effect |
---|---|---|---|---|---|---|
100 series | Hydrophilic | ○ | ◎ | ◎ | ||
200 series | Hydrophobic | ◎ | ◎ | ◎ | ◎ | ◎ |
400 series | Anion | ◎ | ○ | |||
500 series | Cation | ○ | ||||
700 series | Hydrogen bond | ○ | ||||
800 series | Hydrophilic Hydrophobic |
◎ | ◎ | ○ | ◎ | |
1000 series | Hydrophobic | ◎ | ◎ | ◎ | ◎ | ◎ |
1200 series | Hydrophobic | ○ | ○ | ○ | ○ | |
1300 series | Hydrophobic | ○ | ○ | ○ | ○ |
◎ : Best
○ : Good
Index
- Basic properties of LIPIDURE®
- -Blocking effect
- -Protein-stabilization effect
- -Sensitizing effect
- Application of Biolipidure®
- -Latex agglutination
- -Immunochromatography
- -ELISA / CLEIA
Products
[Polymer type] Biolipidure® series

Product name | Characteristics | Form |
---|---|---|
Biolipidure®-103 | Hydrophilic side chain | 5 wt% aqueous solution |
Biolipidure®-203 | Hydrophobic side chain | 5 wt% aqueous solution |
Biolipidure®-206 | Hydrophobic side chain | 5 wt% aqueous solution |
Biolipidure®-405 | Anionic side chain | 5 wt% aqueous solution |
Biolipidure®-502 | Cationic side chain | 5 wt% aqueous solution |
Biolipidure®-702 | Hydrogen bonding side chain | 5 wt% aqueous solution |
Biolipidure®-802 | Hydrophobic/hydrogen bonding side chain | 5 wt% aqueous solution |
Biolipidure®-1002 | Hydrophobic side chain | 5 wt% aqueous solution |
Biolipidure®-1201 | Hydrophobic side chain | 5 wt% aqueous solution |
Biolipidure®-1301 | Hydrophobic side chain | 5 wt% aqueous solution |
[Zwitterionic surfactant type] LIPIDURE®-SF08
The LIPIDURE®-SF08 is a low molecule additive for diagnostic reagents.

Product name | CMC [wt%] | Form |
---|---|---|
LIPIDURE®-SF08 | 0.1 | 5 wt% aqueous solution |
Basic performance of Biolipidure®
Blocking effect
Biolipidure® suppresses non-specific adsorption of biomolecule. NOF provides a set of products that can coat the surface of plastic, metal or other various materials.
Biolipidure® consists of wholly synthesized polymers, so it is free from biohazardous risk and has little lot-to-lot variation, making Biolipidure® the best blocking agent for diagnostic reagents.

Example 1: Blocking microplates
After a microplate was treated with Biolipidure®, the amount of non-specific adsorption of enzyme-labeled antibody was measured. The amount of non-specific adsorption of enzyme-labeled antibody was significantly reduced, proving that Biolipidure® provides excellent blocking effect.


[Test procedure]
- 200 µL of LIPIDURE® (diluted 10-fold by PBS) was applied to a 96-well plate.
- The plate was aspirated and dried overnight.
- 100 µL of 24,000-fold diluted HRP-IgG was added.
- After 1 hr incubation at a room temperature, the plate was washed with PBS-T.
- TMB colorimetric substrate (KPL) was applied and the non-specific adsorption of HRP-IgG was measured.
Example 2: Blocking magnetic beads
After magnetic beads was treated with Biolipidure®, the amount of non-specific adsorption of enzyme-labeled antibody was measured.
The amount of non-specific adsorption of enzyme-labeled antibody was significantly reduced, showing that Biolipidure® provides excellent blocking effect.


[Test procedure]
- 170µg/mL of magnetic beads suspension was added to the same amount of Biolipidure® diluted 5-fold by TBS.
- After 1 hr of incubation at a room temperature, the beads was washed with TBS.
- 10 ng of AP-IgG was added.
- After 1 hr of incubation at a room temperature, the beads was washed with TBS–T.
- Chemiluminescent substrate was applied and non-specific adsorption of AP-IgG was measured.
Example 3: Blocking magnetic beads (Antibody solution)
The same protein-blocking effect was also achieved when Biolipidure® was added to an antibody solution. Biolipidure® was added to a diluted solution of enzyme-labeled antibody, and the amount of non-specific adsorption to magnetic beads was measured.
The amount of non-specific adsorption of enzyme-labeled antibody was significantly reduced, proving that LIPIDURE® provides excellent blocking effect.
* Biolipidure® does not affect antibody titer.


[Test procedure]
- 0.1 times volume of Biolipidure® added to 100 ng/mL of AP-IgG solution.
- 100 µL of the AP-IgG solution containing Biolipidure® was added to 8.6 µg of magnetic beads.
- After 1 hr of incubation at a room temperature, the beads was washed with TBS-T.
- Chemiluminescent substrate was applied and non-specific adsorption of AP-IgG was measured.
Protein-stabilization effect
Biolipidure® stabilizes proteins, probably because the interaction between Biolipidure® and proteins maintains the native state structure of the proteins.

Example 1: Stabilization of antibodies (Solid phase)
The stability of immobilized capture antibody was improved by treating the surface od the microplate by Biolipidure®. This treatment also reduces the non-specific adsorption of proteins applied subsequently.
After the capture-antibody-immobilized microplate was coated with Biolipidure® and stored at 25°C, the residual antibody titer was measured using ELISA (sandwich method).
By using Biolipidure®, the stability of antibody was improved, that is, alsmost 100% of antibody titer was maintained for more than 15 days.


[Test procedure]
- Anti-mouse IgG antibody (capture antibody) was immobilized on a microplate.
- After 1 hr of incubation at a room temperature, the plate was washed with PBS.
- 200 µL of LIPIDURE® diluted 10-fold by PBS was added.
- After 1 hr of incubation at a room temperature, the plate was washed with PBS.
- The microplate was stored at 25°C in desiccator.
- Mouse IgG (antigen) was added.
- After 1 hr of incubation at a room temperature, the plate was washed with PBS-T.
- HRP labeled anti-mouse IgG antibody was added.
- After 1 hr of incubation at a room temperature, the plate was washed with PBS-T.
- Colorimetric substrate (TMB) was added and the antibody titer of the capture antibody was measured.
Example 2: Stabilization of antibodies (Liquid phase)
LIPIDURE®-SF provides stabilization effect when added to a diluted solution of enzyme-labeled antibody.
LIPIDURE®-SF was added to a diluted solution of enzyme-labeled antibody and residual enzyme activity was measured after storage at 4°C.
By adding LIPIDURE®-SF, the stability of enzyme-labeled antibody was markedly improved and 100% of enzyme activity was maintained for 60 days.
* LIPIDURE®-SF does not affect antibody titer.


[Test procedure]
- 0.02 times volume of LIPIDURE®-SF08 was added to 10,000-fold diluted solution of enzyme-labeled antibody.
- The solution was stored at 4°C.
- The residual activity of labelled enzyme was measured with colorimetric substrate (TMB).
Sensitizing effect
BIOLIPIDURE® exhibits sensitizing effect in immunoassay systems such as latex agglutination or immunochromatography, most likely caused by the improved dispersion of latex particle or gold colloid.
Example 1: Sensitizing effect of Biolipidure® in latex agglutination test
Biolipidure® provides excellent sensitizing effect in latex agglutination test.
[Test procedure]

(Detection was performed using an automated analyzer.)

Example 2: Sensitizing effect of Biolipidure® in lateral flow test (immunochromatography)
Biolipidure® provides excellent sensitizing effect in lateral flow test (immunochromatography).
[Test procedure]

(The immunochromatography kit is manufactured in our laboratory.)

Application examples using Biolipidure®
Latex agglutination test
Biolipidure® provides excellent sensitizing effect in latex agglutination test.
[Test procedure]

(Detection was performed using an automated analyzer.)

Lateral flow test (Immunochromatography)
Biolipidure®, especially Biolipidure-40X, provides improved sensitizing effect in lateral flow test (immunochromatography).
Dengue virus kit
[Test procedure]


Adenovirus kit
[Test procedure]


ELISA / CLEIA
Biolipidure® provides sensitizing effect in sandwich type Chemiluminescent Enzyme Immunoassay (CLEIA).


[Test procedure]
- Mouse anti-Human IgG antibody was immobilized on a microplate.
- After 1 hr of incubation at a room temperature, the plate was washed with TBS.
- Biolipidure® solution (0.1 times volume) was added.
- The plate was incubated for 1 hr at a room temperature.
- Human IgG (antigen) standard was added.
- After 1 hr of incubation at a room temperature, the plate was washed with TBS-T.
- AP labeled anti-Human IgG antibody was added.
- After 1 hr of incubation at a room temperature, the plate was washed with TBS-T.
- Chemiluminescent substrate was added and calibration curve for Human IgG concentration was prepared.