Materials for Oral Care

LIPIDURE^® has the ability to prevent the adhesion of bacteria that cause dental caries and can protect mucosal cell from the toxicity of periodontal disease bacteria. Furthermore, LIPIDURE^® can suppress bitterness.

Suppressing the adhesion of bacteria that cause dental caries (S. mutans) using LIPIDURE^®

LIPIDURE^® suppresses the adhesion of bacteria that cause dental caries.

[Test procedure]

1. Treated hydroxyapatite plate with saliva and washed it with water.
2. Dipped the plate into 1 mL of LIPIDURE^® and 1 mL of water for 30 seconds or 10 minutes.
3. Washed the plate with water again.
4. Added precultured S. mutans, which was incubated at 37°C in anaerobic condition, and washed the plate with saline.
5. Observed S. mutans that have adhered to the hydroxyapatite plate using scanning electron microscope (SEM) and measured the number of bacteria.

The top figure above shows that applying the LIPIDURE^® treatment to a hydroxyapatite plate for more than 30 seconds suppresses the formation of biofilm to about 40%.
Bacteria that cause dental caries produces biofilm for control, but the bottom figure shows that applying the LIPIDURE^® treatment for more than 30 seconds reduces the formation of biofilm.

Protecting mucosal cell from the toxicity of periodontal disease bacteria using LIPIDURE^®
LIPIDURE^® protects the mucosal cell from the toxicity of periodontal disease bacteria.

[Test procedure]

1. Cultured gingival epithelial cells.
2. Dipped the cell into LIPIDURE^® and saline for 30 seconds.
3. Washed it with saline again.
4. Added the toxin of periodontal disease bacteria (PgLPS).
5. Measured the interleukin-8 (IL-8).

A study conducted by a group led by Prof. Yoichiro Miyake of Tokushima University.

A higher bar indicates higher toxicity. As the concentration of LIPIDURE^® changes from blue to yellow, the toxicity decreases.

Suppressing bitterness using LIPIDURE^®

[Test procedure]

1. Kept the test drug (containing LIPIDURE^®) or control drug (not containing LIPIDURE^®) in the subject’s mouth, who gargled and spit out the solution.
2. Conducted a questionnaire survey about the taste during and after gargling.
   ^* Subjects were blindfolded during the testing to prevent them from distinguishing the sample.

LIPIDURE^® has the ability to suppress bitterness.

The pink bar indicates the number of subjects who answered that the study drug containing LIPIDURE^® is less bitter. The test was performed with a solution containing 0.3% of LIPIDURE^®.

Materials for Topical use

LIPIDURE^® has excellent skin absorptivity and moisturizing effect, in addition to the ability to protect skin from irritants.

Adsorbing three dimensional cultured skin model using LIPIDURE^®
LIPIDURE^® has shown that it is able to adsorb three dimensional cultured skin model.

The top figure above shows the LIPIDURE^® treatment to three dimensional cultured skin model.
The figure on the bottom left shows the cut section of the three dimensional cultured skin model (bright-field image), while the bottom right shows an image of the differential interference.

In the figure on the bottom right, the LIPIDURE^® film on three dimensional cultured skin model turns white and shows that the LIPIDURE^® was adsorbed into skin model.

Hygroscopicity and moisture-retaining property of LIPIDURE^®
LIPIDURE^® becomes less sticky after adsorbing moisture and shows excellent moisture-retaining property.

Moisture-retaining property of LIPIDURE^® for skin
LIPIDURE^® stays on the skin after it is washed with water and shows excellent moisture-retaining property.

[Test procedure]

1. Measured the water content in the stratum corneum before applying the sample.
2. Measured the water content in the stratum corneum after applying LIPIDURE^® and sodium hyaluronate solution (0.2% in terms of pure content for both samples).
3. Washed the applied area with water.
4. Measured the water content in the stratum corneum of the applied area after one hour.

The moisture-retention ability was 1.0 before application and approximately 1.5 after application for both samples. After washing with water, the moisture-retention ability decreased to around 1.0 for sodium hyaluronate solution, while the moisture retention ability increased to around 2.2 for LIPIDURE^®.

Alleviating irritation using LIPIDURE^®
LIPIDURE^® has the ability to alleviate irritation.

In the bottom figure, significant erythema was identified for AHA only, but erythema disappeared for LIPIDURE^®+AHA.

Conference presentation and reference
Hirota, K., Yumoto, H., Miyamoto, K., Yamamoto, N., Murakami, K., Hoshino, Y., Matsuo, T., and Miyake, Y. (2011). J. Dent.Res., 90 (7), 900-905.
Yumoto, H., Hirota, K., Hirao, K., Miyazaki, T., Yamamoto, N., Miyamoto, K., Murakami, K., Fujiwara, N., Matsuo, T., and Miyake, Y. (2015).
J. Biomed. Mater. Res. Part A, 103(2), 555-563.