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The structure of Biolipidure®  |
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| Protection of proteins |
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Suppression of non-specific adsorption |
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- Biolipidure® having phospholipid polar groups (Figure 1), 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers, are well known for the suppression of protein adsorption1 and denaturation on the surface.2
- In previous articles, water-soluble MPC polymer stabilizes the HRP-IgG conjugate3 and the MPC polymer is superior to proteins as a blocking reagent.4
- Biolipidure® works as a blocking reagent, a stabilizer and an enhancer for Immunoassay.
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| 1) |
K. Ishihara, N. P. Ziats, B. P. Tierney, N. Nakabayashi and J. M. Anderson, J.Biomed.Mater.Res., 25, 1397 (1991) |
| 2) |
K. Ishihara, H. Nomura, T. Mihara, K. Kurita, Y. Iwasaki and N. Nakabayashi, J.Biomed.Mater.Res., 39, 323 (1998) |
| 3) |
S. Sakaki, N. Nakabayashi and K. Ishihara, J.Biomed.Mater.Res., 47, 523 (1999) |
| 4) |
S. Sakaki, Y. Iwasaki, N. Nakabayashi and K. Ishihara, Polym.J., 32, 637 (2000) |
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The structure of Biolipidure®  |
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Features
- No danger of biohazards
- No difference in lots
- Suppression of non-specific adsorption
- Stabilization of immobilized antibody
- Stabilization of enzyme-antibody conjugate
- Enhancement of aggregation reaction
- Enhancement of enzyme-substrate reaction
- Enhancement of accuracy
Reduction of CV value ([standard deviation] / [average] x 100)
- Improvement of particle dispersiveness
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Reduction of CV value |
Improvement of sensitivity in turbidimetric assay |
Suppression of non-specific adsorption |
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General Usage
- Can be supplied as 5 wt% aqueous solution
Do not use Biolipidure® directly, because it is not a buffer solution.
- Can be used in ELISA, Western Blotting, Immuno-histochemistry.
- Dilute with a buffer solution.
It is recommended that the conc. of Biolipidure® is used as 1.0 wt% - 0.01 wt%.
- Store at 4°C.
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| Name |
Property * |
Blocker |
Stabilizer |
Enhancer |
Vol. |
| Biolipidure-103 |
Amphoteric |
- |
Good |
Very Good |
10mL |
| Biolipidure-203 |
Amphoteric |
Good |
Good |
Very Good |
10mL |
| Biolipidure-206 |
Amphoteric |
Very Good |
Very Good |
Good |
10mL |
| Biolipidure-405 |
Anionic |
- |
- |
Very Good |
10mL |
| Biolipidure-502 |
Cationic |
- |
- |
Good |
10mL |
| Biolipidure-702 |
Amphoteric |
- |
- |
Good |
10mL |
| Biolipidure-802 |
Amphoteric |
Very Good |
Very Good |
- |
10mL |
| Biolipidure-1002 |
Amphoteric |
Very Good |
Good |
Good |
10mL |
| Biolipidure-1201 |
Amphoteric |
Good |
- |
- |
10mL |
| Biolipidure-1301 |
Amphoteric |
Good |
- |
- |
10mL |
| Set of all Biolipidure |
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1 set |
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Note
* Hydrophilicity :103 > 405, 502, 702 > 203, 802, 1201, 1301 > 206, 1002 |
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Blocking |
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Solid phase: 96-well plate (Maxisorp F96, Nunc)
Immobilized antibody: anti (mouse IgG)IgG
Antigen: non
Enzyme-IgG conjugate: HRP-Anti (mouse IgG) IgG conjugate
Substrate: o-phenylenediamine dihydrochloride
Detection: 492 nm |
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Figure 2. The amount of non-specific adsorbed HRP-IgG conjugate |
Experimental condition:
- Immobilization of anti (mouse IgG)IgG
- Blocking by 0.1% (1 mg/mL) Biolipidure® and 1 mg/mL BSA
- Dispensation of HRP-Anti (mouse IgG) IgG conjugate
- Dispensation of o-phenylenediamine dihydrochloride
- Measurement of absorbance at 492 nm
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Stabilization of immobilized antibody |
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Solid phase: 96-well plate (Maxisorp F96, Nunc)
Immobilized antibody: anti (mouse IgG)IgG
Antigen: mouse IgG
Enzyme-IgG conjugate: HRP-Anti (mouse IgG) IgG conjugate
Substrate: o-phenylenediamine dihydrochloride
Detection: 492 nm |
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Figure 3. Remaining activities of immobilized antibody that was stored at 40°C |
Experimental condition:
- Immobilization of anti (mouse IgG)IgG
- Blocking by by 0.1% Biolipidure® and 0.1 % BSA
- Storage at 40°C : 0, 4, 7, 14 and 20 day
- Dispensation of mouse IgG
- Dispensation of HRP-Anti (mouse IgG) IgG conjugate
- Dispensation of o-phenylenediamine dihydrochloride
- Measurement of absorbance at 492 nm
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Stabilization of HRP-IgG conjugate |
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Solid phase: 96-well plate (Maxisorp F96, Nunc)
Immobilized antibody: anti (mouse IgG)IgG
Antigen: mouse IgG
Enzyme-IgG conjugate: HRP-Anti (mouse IgG) IgG conjugate
Substrate: o-phenylenediamine dihydrochloride
Detection: 492 nm |
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Figure 4. Remaining activities of HRP-IgG conjugate that was stored at 25°C |
Experimental condition:
- Preparation of plate (immobilized antibody and reacted antigen)
- Dispensation of 1 % Biolipidure® and BSA into HRP-IgG conjugate
- Storage at 25°C: 0, 3, 7, 14, 24 and 37 day
- Dispensation of HRP-Anti (mouse IgG) IgG
- Dispensation of o-phenylenediamine dihydrochloride
- Measurement of absorbance at 492 nm
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Enhancement of aggregation reaction |
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Analysis: 0.8 mg/dL CRP using 7070 Auto Analyzer (HITACH)
Measurement: latex turbidimetric immunoassay |
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Figure 5. Enhancement of latex aggregation |
Experimental condition:
- Dispensation of 1% Biolipidure®, PEG and PVP into R1 reagent
PEG: polyethylene glycol (Mw = 6,000)
PVP: polyvinylpyrrolidone (Mw = 40,000) |
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Effect of Biolipidure® for immunochromatography |
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* Biolipidure®-405 is acidic so you need to control the PH of your product. |
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Usage of Biolipidure® as a mobile phase
#1 Prepare the buffer solution including one wt% of Biolipidure®.
#2 Dissolve the sample (For example; mucosa) in the above buffer.
#3 Put the diluted sample on the solid phase of immunochromatography. |
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Usage of Biolipidure® as a solid phase
#1 Prepare the solution of antibodies-conjugated gold colloids.
#2 Draw the line using the colloids solution on the solid phase of immunochromatography.
#3 Soak the solid phase in the buffer solution including one wt% of Biolipidure®.
#4 Pick up the solid phase and dry it. You can get the solid phase blocked by Biolipidure®. |
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