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Features
- Hemo-compatibility
- Anti-adhesion of proteins and cells
- Anti-denaturing of proteins
- Anti-activation of cells
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Usage
- Dissolve Lipidure®-CM in ethanol and prepare a 0.5wt% ethanol solution.
- Dip the base material into the coating solution for 1 min.
- Dry the coated material at room temperature or one hour at 50°C.
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Substrates
- Lipidure®-CM can be coated on the following substrates:
Plastics (PET, PMMA, PSt, PU, PC, PE, PP)
Glass, Ceramics and Metals (SUS, titanium, gold)
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Structure of Lipidure®-CM |
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Comparison of results |
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Protein Adsorption |
Experimental procedure
- Dispensation of HRP-IgG conjugate solution into each well
- Incubation for 1 h at 25°C
- Washing three times with Dulbeco’s PBS including 0.05% of Tween20.
- Dispensation of 3,3’,5,5’-tetramethylbenzidine solution
- Measurement of absorbance at 450 nm
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Lipidure treatment*1 : 96 wells PSt plate treated by Lipidure®-CM5206 |
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The Lipidure® treatment was able to reduce the amount of IgG absorption to below 10% of non-treated pleat. The same results were observed with other proteins.
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Macrophage adhesion |
Experimental procedure
- Seeding of macrophage into each well (3.3 x 105 cells/well)
- Incubation for 1 h at 37°C in 5% CO2 atmosphere
- Washing with Dulbeco’s PBS
- Fixing with glutaraldehyde solution
- Observation by SEM
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Lipidure treatment*2: 24 wells PSt plate treated by Lipidure®-CM5206 |
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The activation and adhesion of macrophage was prevented by Lipidure® treatment. Also, the Lipidure® treatment was effective for other cells. |
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Coating
PC surface can be made by coating with Lipidure®-CM.
The following figure shows the relationship between “the number of times the coating was applied” and “the thickness of the coating film”.
The thickness of the Lipidure®-CM coating film is calculated by weight measurement.
These data are examples in the case of dip coating. The thickness was controlled by adjusting the number of times of a coating was applied and the concentration of Lipidure® in the coating solution. |
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Safety Data
We have the following toxicological data for Lipidure®-CM5206.
Acute oral toxicity, Skin irritation, Skin sensitization, Mutagenic potential (Ames Test), Hemolysis test, Endotoxin test |
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Relationship between “number of times coating was applied” and “thickness of coating film” |
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