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Anti 4-HNE (4-Hydroxy-2-nonenal) Monoclonal Antibody |
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4-HNE[4-hydroxy-2-nonenal] is an oxidized secondary product (aldehyde) which is formed when organic lipids consisting of polyunsaturated fatty acid of the ƒÖ6 group, such as arachidonic acid, receive oxidization stress. 4-HNE forms a relatively stable reactant (Michael adduct) with proteins. This antibody is highly specific to the stable 4-HNE-protein compound. |
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| Immunogen |
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Antibody obtained by cultivating hybridoma, which is formed by fusing the spleen cell of a BALB/c mouse sensitized with HNE modification protein and the myeloma cell of a mouse, in the abdominal cavity of the BALB/c mouse. |
| Form/Shape |
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2 types of PBS solution, 20μg/vial and 100μg/vial (100μg/mL)
(0.01M Phosphoric Acid Buffer Solution, 0.1% NaN3 and 1% BSA contained)
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| Specificity |
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Combines specifically with HNE modification protein. |
| Class |
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IgG 1 (ƒÈ) |
| Volume |
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20 or 100μg/vial (100μg/mL) |
| Storing Method |
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Stored at -20 degrees centigrade |
| Package |
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2ml Vial |
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Western Blot:
Elapsing Time after administration of Iron Nitrilotriacetic Acid 15mg Fe/kg,
12.5% Polyacrylamidegel
Electrophoresis (each lane, 40μg albumen),
Anti 4-HNE Monoclonal Antibody
2.15μg/ml, ECL)
(Provided by Dr. Toyokuni at Kyoto University)
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Immunohistochemistry:
Upper: Rat kidney 3 hours
after administration of Iron
Nitrilotriacetic Acid 15mg
Fe/kg
Lower: Controlled rat kidney
(Anti 4-HNE Monoclonal
25μg/ml, ABC Method)
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| Product Number |
Product Name |
Package |
Price (without tax) |
| N213220 |
Anti 4-HNE Antibody |
20μg |
¥18,000- |
| N213210 |
Anti 4-HNE Antibody |
100μg |
¥70,000- |
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